Development of a multi-biomarker approach of genotoxicity on the three spined stickleback for aquatic biomonitoring application - Ineris - Institut national de l'environnement industriel et des risques Access content directly
Conference Papers Year : 2022

Development of a multi-biomarker approach of genotoxicity on the three spined stickleback for aquatic biomonitoring application

Amélie Cant
Jean Marc Porcher
  • Function : Author
  • PersonId : 832628
Jean Prygiel
  • Function : Author
Audrey Catteau
  • Function : Author
Laurence Delahaut
  • Function : Author
Alain Geffard

Abstract

The aquatic environment is considered as the receptacle of anthropogenic pressures, including chemical contamination as genotoxic compounds, which can impact the health of aquatic species. Genotoxic substances may directly or indirectly affect the integrity of cell’s genetic material, which can lead to long-term mutagenic, carcinogenic or teratogenic effects. In this context, the measure of damages at different genomic scales may be relevant and provide an integrated view of the genotoxic risk of exposed organism. The finality of our work is to propose their use as early-warning signals of potential long-term alterations to population and ecosystem health. Genotoxicity assessment was performed on erythrocytes of the three- spined stickleback by combining the measure of DNA integrity by the alkaline and Fpg (formamidopyrimidines DNA glycosylase)-modified comet assays and the measure of variations in nuclear DNA content by flow cytometry (FCM). In this sense, blood cells of fish offer many advantages as cellular models as their ease of collection, gives a high density of erythrocytes already dissociated allowing to obtain multiple assays on the same sample. The alkaline comet assay has been largely used with erythrocytes of fish to detect alkali-labile sites, DNA-double- and single- strand breaks. The Fpg-modified comet assay has been optimized in the present study, which expand the comet assay sensitivity to oxidized pyrimidines. Furthermore, FCM may be applied to assess the variations in the nuclear DNA content of a large population of cells and has already demonstrated its relevance for detecting chromosomal damages in blood cells of fish. The presentation will detail results regarding the definition of baseline levels of genotoxicity biomarkers (DNA strand breaks, Fpg-sensitive sites, DNA content, erythrocyte mortality), which are useful in an ecotoxicological context. These biomarkers have been measured in various field context (active biomonitoring) and integrated in the existing multi-biomarker approach of the three-spined stickleback (Gasterosteus aculeatus L., 1758).

Domains

Toxicology
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Dates and versions

ineris-03988060 , version 1 (14-02-2023)

Identifiers

  • HAL Id : ineris-03988060 , version 1

Cite

Amélie Cant, Anne Bado-Nilles, Jean Marc Porcher, Jean Prygiel, Audrey Catteau, et al.. Development of a multi-biomarker approach of genotoxicity on the three spined stickleback for aquatic biomonitoring application. 10. International Conference on Stickleback Behaviour and Evolution, Jul 2022, Hólar, Iceland. ⟨ineris-03988060⟩
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