Characterization of the nano-bio interaction between metallic oxide nanomaterials and freshwater microalgae using flow cytometry - Ineris - Institut national de l'environnement industriel et des risques Access content directly
Journal Articles Nanotoxicology Year : 2020

Characterization of the nano-bio interaction between metallic oxide nanomaterials and freshwater microalgae using flow cytometry

Abstract

Since nanomaterials (NMs) are particulate contaminants, their first contact with organisms is a physical encounter ruled by physic-chemical processes that can determinate the potential NMs accumulation, toxicity, and trophic transfer. Freshwater ecosystems often become a final depository for NMs, so they can get in contact with the biota, especially primary organisms as algae. There are almost none comparative studies of this interaction using various NMs in the same conditions. This work identifies, analyzes, and compares the algae-NMs interaction by flow cytometry after a short-term contact test in which three freshwater algae (Raphidocelis subcapitata,Desmodesmus subspicatus,andChlorella vulgaris)interact individually with a set of twelve metallic oxide NMs. Dose-response profiles and differences in the algae-NMs interaction were found according to each algae species (C. vulgarishad the most affinity, starting the interaction from 0.5 mg/L andD. subspicatushad the less affinity starting at 5 mg/L). Flow cytometry results were confirmed by optical microscopy. Some NMs characteristics were identified as key-factors that govern the algae-NMs interaction: NMs composition (no interaction for SiO2NMs), surface electric charge (higher interaction for the positively charged NMs and lower interaction for the negatively charged ones) and crystalline form (for TiO2NMs). The presented method can be useful for a rapid determination of the interaction between free cells organisms as microalgae and (nano)particulate substances.
Fichier principal
Vignette du fichier
2020-079_post-print.pdf (887.63 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

ineris-03318327 , version 1 (09-08-2021)

Identifiers

Cite

Andrea Rivero Arze, Nicolas Manier, Amelie Chatel, Catherine Mouneyrac. Characterization of the nano-bio interaction between metallic oxide nanomaterials and freshwater microalgae using flow cytometry. Nanotoxicology, 2020, 14 (8), pp.1082-1095. ⟨10.1080/17435390.2020.1808106⟩. ⟨ineris-03318327⟩
98 View
154 Download

Altmetric

Share

Gmail Facebook X LinkedIn More