Use of transepithelial electrical resistance in the study of pentachlorophenol toxicicity - Ineris - Institut national de l'environnement industriel et des risques Access content directly
Conference Papers Year : 1998

Use of transepithelial electrical resistance in the study of pentachlorophenol toxicicity

Abstract

The toxicity of pentachlorophenol (PCP), a polluting substance believed to exert a narcotic effect, was assayed using the Caco-2 cell line as a model. In order to assess this toxicity as fully as possible, several viability tests, each examining different endpoints, have been used. Neutral red uptake was found to be more sensitive to PCP than MTT and Alamar Blue tests. Transepithelial electrical resistance (TEER) was shown to be the most sensitive to PCP at concentrations and exposure times where the Alamar Blue, LDH leakage and Blue Dextran passage did not evidence any effect. Blue Dextran passage and optical microscopy revealed cellular detachment at concentrations where LDH and Alamar Blue showed little or no cytotoxicity. Thus, PCP seems to affect the integrity of the intestinal barrier at levels where no cytotoxicity is seen. Our results support the notion that TEER can be used as a very sensitive method for evaluating membrane-perturbing toxicants
No file

Dates and versions

ineris-00969694 , version 1 (02-04-2014)

Identifiers

  • HAL Id : ineris-00969694 , version 1
  • INERIS : PU-1998-052a

Cite

G. Velarde, Selim Ait-Aissa, Chantal Gillet, Françoise Rogerieux, Claude Lambre, et al.. Use of transepithelial electrical resistance in the study of pentachlorophenol toxicicity. 10. International Workshop on In Vitro Toxicology (INVITOX 1998), Sep 1998, Winchester, United Kingdom. ⟨ineris-00969694⟩

Collections

INERIS
23 View
0 Download

Share

Gmail Facebook X LinkedIn More