Improving Quality in Nanoparticle-Induced Cytotoxicity Testing by a Tiered Inter-Laboratory Comparison Study - Ineris - Institut national de l'environnement industriel et des risques Access content directly
Journal Articles Nanomaterials Year : 2020

Improving Quality in Nanoparticle-Induced Cytotoxicity Testing by a Tiered Inter-Laboratory Comparison Study

Inge Nelissen
  • Function : Author
Andrea Haase
  • Function : Author
Sergio Anguissola
  • Function : Author
Louise Rocks
  • Function : Author
An Jacobs
  • Function : Author
Hanny Willems
  • Function : Author
Christian Riebeling
  • Function : Author
Andreas Luch
  • Function : Author
Jean-Pascal Piret
  • Function : Author
Olivier Toussaint
  • Function : Author
A.C. Gutleb
  • Function : Author
Servane Contal
  • Function : Author
Silvia Diabate
  • Function : Author
Carsten Weiss
  • Function : Author
Tamara Lozano-Fernandez
  • Function : Author
Africa Gonzalez-Fernandez
  • Function : Author
Maria Dusinska
  • Function : Author
Anna Huk
  • Function : Author
Vicki Stone
  • Function : Author
Nilesh Kanase
  • Function : Author
Marek Nocun
  • Function : Author
Maciej Stepnik
Stefania Meschini
  • Function : Author
Maria Grazia Ammendolia
  • Function : Author
Nastassja Lewinski
  • Function : Author
Michael Riediker
  • Function : Author
Marco Venturini
  • Function : Author
Federico Benetti
  • Function : Author
Jan Topinka
  • Function : Author
Tana Brzicova
  • Function : Author
Silvia Milani
  • Function : Author
Joachim Radler
  • Function : Author
Anna Salvati
  • Function : Author
Kenneth A. Dawson
  • Function : Author

Abstract

The quality and relevance of nanosafety studies constitute major challenges to ensure their key role as a supporting tool in sustainable innovation, and subsequent competitive economic advantage. However, the number of apparently contradictory and inconclusive research results has increased in the past few years, indicating the need to introduce harmonized protocols and good practices in the nanosafety research community. Therefore, we aimed to evaluate if best-practice training and inter-laboratory comparison (ILC) of performance of the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay for the cytotoxicity assessment of nanomaterials among 15 European laboratories can improve quality in nanosafety testing. We used two well-described model nanoparticles, 40-nm carboxylated polystyrene (PS-COOH) and 50-nm amino-modified polystyrene (PS-NH2). We followed a tiered approach using well-developed standard operating procedures (SOPs) and sharing the same cells, serum and nanoparticles. We started with determination of the cell growth rate (tier 1), followed by a method transfer phase, in which all laboratories performed the first ILC on the MTS assay (tier 2). Based on the outcome of tier 2 and a survey of laboratory practices, specific training was organized, and the MTS assay SOP was refined. This led to largely improved intra- and inter-laboratory reproducibility in tier 3. In addition, we confirmed that PS-COOH and PS-NH2 are suitable negative and positive control nanoparticles, respectively, to evaluate impact of nanomaterials on cell viability using the MTS assay. Overall, we have demonstrated that the tiered process followed here, with the use of SOPs and representative control nanomaterials, is necessary and makes it possible to achieve good inter-laboratory reproducibility, and therefore high-quality nanotoxicological data.
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Dates and versions

ineris-03230216 , version 1 (19-05-2021)

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Inge Nelissen, Andrea Haase, Sergio Anguissola, Louise Rocks, An Jacobs, et al.. Improving Quality in Nanoparticle-Induced Cytotoxicity Testing by a Tiered Inter-Laboratory Comparison Study. Nanomaterials, 2020, 10 (8), pp.1430. ⟨10.3390/nano10081430⟩. ⟨ineris-03230216⟩
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