%0 Conference Paper %F Oral %T Pulmonary responses to silica dust : the influence of crystalline structures and physico-chemical properties %+ Institut National de l'Environnement Industriel et des Risques (INERIS) %A Honnons, Sylvie %A Oberson, Dominique %A Wastiaux, Agnès %A Dzwigaj, S. %A Pezerat, H. %A Volante, Marco %A Fubini, B. %A Porcher, Jean-Marc %< avec comité de lecture %B 2. International Symposium on Silica Silicosis & Cancer %C San Francisco, United States %8 1993-10-28 %D 1993 %Z Environmental SciencesConference papers %X The inhalation of silica crystalline polymorphs leads to the development of pulmonary nodules that vary in extent and in fibrotic content. In this study, a single protocol was used to test different silica samples (i.e., 2 non-crystalline samples: one vitreous silica, one diatomaceous earth , 4 crystalline samples: one pure a-quartz ground in a wet atmosphere, one of the same quartz heated until it becomes oc-cristobalite and 2 cristobalites obtained by heating diatomaceous earth). Wistar rats received a single intra-tracheal injection of alveolar dust and were followed over a period of 3 months. The results were exploited using macroscopic examination (wet lung weight), histological examination and biochemical dosing (hydroxyproline, lipid, and silica content). The results showed that vitreous silica, diatomaceous earth, wet-ground quartz and both types of cristobalites are responsible to a greater or lesser extent for the pulmonary reaction typical of exposure to silica (i.e., nodules ); with different cellular and fibrotic surface areas for each sample. a-cristobalite (obtained from the wet-ground quartz heated to a very high temperature; 1,300 °C) leads to the formation of only small foreign-body granulomas. All crystalline minerals tested caused alveolar lipoproteinosis. It was thus concluded that the crystalline structure plays a less important role than the surface state in the formation of silicotic nodules. %G English %2 https://ineris.hal.science/ineris-00971883/document %2 https://ineris.hal.science/ineris-00971883/file/1993-078_hal.pdf %L ineris-00971883 %U https://ineris.hal.science/ineris-00971883 %~ SDE %~ INERIS %~ GIP-BE