With the rise of sustainable development, rehabilitation of brownfield sites located in urban areas has become a major concern. Remediation of contaminated soils is therefore a strong aim with the development of bioremediation and tools for risk assessment. Actually, criteria for landfilling are based on leaching tests that represent the mobilized fraction and therefore may present a risk in case of transfer. However, ecotoxicological tests to guide the landfill are not yet currently used. Nevertheless, their use seems very relevant to assess the actual toxicity of the leachate and better reflects the reality of effects on living organisms. Standard leaching tests (EN 12457-2) asked by European authorities to guide landfilling were first performed on ten samples of soil taken from a site recycling lead-acid batteries. This experiment determines the fraction potentially be mobilized and therefore poses a risk if transferred. Physico-chemical parameters from all samples were also determined (pH, lead total concentrations, etc.). Toxicity of leachate was evaluated by Daphnia magna (OCDE 202) and Alivibrio fischeri (Vibrio fischeri, ISO 11348) bioassays. Besides, four modified Escherichia coli stains with luminescence modulated by heavy metals were also used to assess the toxicity of these pollutants. Results shown that although total lead concentrations can be very high (up to 40 000 mg / kg), only a small fraction was solubilized. Chemical analysis and ecotoxicological bioassays were well correlated. This study highlighted the use of modified bacteria strains sensitive to metals will be useful tools for the end-users for environmental monitoring of contaminated sites.