Characterization of testicular expression of P450 17alpha-hydroxylase, 17,20-lyase in zebrafish and its perturbation by the pharmaceutical fungicide clotrimazole - Ineris - Institut national de l'environnement industriel et des risques Access content directly
Journal Articles General and Comparative Endocrinology Year : 2011

Characterization of testicular expression of P450 17alpha-hydroxylase, 17,20-lyase in zebrafish and its perturbation by the pharmaceutical fungicide clotrimazole

Abstract

The aim of the present study was to characterize P450 17alpha-hydroxylase/17,20-lyase (cyp17a1) expression in zebrafish and to assess the effect of the pharmaceutical clotrimazole, a known inhibitor of various cytochrome P450 enzyme activities, on testicular gene and protein expression of this enzyme as well as on the testicular release of 11-ketotestosterone (11-KT), a potent androgen in fish. We first showed that cyp17a1 is predominantly expressed in gonads of zebrafish, notably in male. In vivo, clotrimazole induced a concentration-dependent increase of cyp17a1 gene expression and Cyp17-I protein synthesis in zebrafish testis. Using zebrafish testicular explants, we further showed that clotrimazole did not directly affect cyp17a1 expression but that it did inhibit 11-KT release. These novel data deserve further studies on the effect of azole fungicides on gonadal steroidogenesis.
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ineris-00961766 , version 1 (20-03-2014)

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Nathalie Hinfray, Damien Baudiffier, Marcello C. Leal, Jean-Marc Porcher, Selim Ait-Aissa, et al.. Characterization of testicular expression of P450 17alpha-hydroxylase, 17,20-lyase in zebrafish and its perturbation by the pharmaceutical fungicide clotrimazole. General and Comparative Endocrinology, 2011, 174 (3), pp.309-317. ⟨10.1016/j.ygcen.2011.09.008⟩. ⟨ineris-00961766⟩
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